Actividad antioxidante del extracto hidroalcohólico de hojas de Vallesia glabra (Cav.) Link. (agracejo peruano) usando el radical ABTS, Lima 2026
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Universidad Privada de Huancayo Franklin Roosevelt
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El objetivo fue evaluar la actividad antioxidante del extracto hidroalcohólico de hojas de Vallesia glabra (Cav.) Link. frente al radical ABTS. La investigación fue básica, cuantitativa, experimental in vitro, prospectiva y transversal, utilizando 100 g de hojas secas maceradas con etanol al 70 %. El extracto fue evaluado mediante tamizaje fitoquímico y ensayo ABTS por espectrofotometría UV-Vis a 734 nm, empleando Trolox como patrón. Se obtuvo una inhibición máxima de 76,66 % a 500 μg/mL, IC₅₀ de 312,01 μg/mL y TEAC de 9,94 μg TE/mg, con diferencias significativas (p = 0,000). Se concluyó que el extracto presentó actividad antioxidante significativa, asociada principalmente a sus compuestos fenólicos y flavonoides.
The objective was to evaluate the antioxidant activity of the hydroalcoholic extract of Vallesia glabra (Cav.) Link. leaves against the ABTS radical. The research was basic, quantitative, experimental, in vitro, prospective, and cross-sectional, using 100 g of dried leaves macerated with 70% ethanol. The extract was evaluated by phytochemical screening and ABTS assay by UV-Vis spectrophotometry at 734 nm, using Trolox as a standard. A maximum inhibition of 76.66% was obtained at 500 μg/mL, with an IC₅₀ of 312.01 μg/mL and a TEAC of 9.94 μg TE/mg, with significant differences (p = 0.000). It was concluded that the extract exhibited significant antioxidant activity, primarily associated with its phenolic and flavonoid compounds.
The objective was to evaluate the antioxidant activity of the hydroalcoholic extract of Vallesia glabra (Cav.) Link. leaves against the ABTS radical. The research was basic, quantitative, experimental, in vitro, prospective, and cross-sectional, using 100 g of dried leaves macerated with 70% ethanol. The extract was evaluated by phytochemical screening and ABTS assay by UV-Vis spectrophotometry at 734 nm, using Trolox as a standard. A maximum inhibition of 76.66% was obtained at 500 μg/mL, with an IC₅₀ of 312.01 μg/mL and a TEAC of 9.94 μg TE/mg, with significant differences (p = 0.000). It was concluded that the extract exhibited significant antioxidant activity, primarily associated with its phenolic and flavonoid compounds.
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